RKS GENETICS

ROYAL KING GENETICS

Breeder-Documented Reference Benchmark
Aggregates breeder specifications, publicly-reported third-party lab data, and grower-community results.
Not an independent ISO 17025 certificate of analysis for the specific seed lot you receive.

GENETICS REFERENCE

· STABILIZED LINE PROFILE ·

REF ID: CAN-2026-04-RW-0924

CORE LINEGeneration:FEMINIZED PHOTOPERIOD·Line:RKS Reserve Line

Phenotype:RW-Pheno #1 (Citrus-Dominant)

Client / Brand

Royal King Seeds Ltd.

Product Name

Red Widow Feminized

Strain Genetics

Autoflower Hybrid (53/47)

Sample ID

RKS-RW-FEM-0924

Batch / Lot Number

RW-2026-0924

Production Date

2026-03-13

Date Received

2026-04-16

Date of Analysis

2026-04-17, 2026-04-28

♛

RKS Stabilized Line

Feminized · Trait-Locked

✓

Breeder Reference

Documented Line

★

Breeder-Selected Phenotype

RW-Pheno #1 of 13

RKS BREEDER DOCUMENTATION

Batch No. RW-2026-0924 · Core Line · Cycle 53

RKS Breeder Documentation

This reference benchmark documents the upper-percentile phenotype of the Red Widow stabilized line. The breeding criteria for this strain prioritize hybrid balance: no single terpene dominating the profile, trichome density above the cohort minimum, and a tight flower-time window. The reference numbers reflect the chosen phenotype carried forward through stabilization. The line traces to a Red Dragon × White Widow cross, with each generation selected to fix the traits that define Red Widow.

Breeding Objective · RW-Pheno #1

Fix the phenotype expression that defines this strain
Improve resistance to common pathogens under indoor SOP
Maintain commercial yield bands across cycles
Reduce flower-window variance ≤ 5 days within cohort

Royal King Genetics

Breeding Team Documentation
Reference benchmark, not a per-lot COA

Total THC
25.0%
Total CBD
0.10%
Total Terpenes
1.79%
Safety
ALL PASS

Cannabinoid Profile

Method: HPLC-DAD · AOAC 2018.11

Analyte	Result (%)	mg/g	LOQ
THCa	27.56	275.6	0.05
Δ9-THC	0.83	8.3	0.05
CBDa	0.01	0.1	0.05
CBD	0.09	0.9	0.05
CBG	0.42	4.2	0.05
CBC	0.33	3.3	0.05
CBN	0.14	1.4	0.05
Total THC ((THCa × 0.877) + Δ9-THC)	25.0	250.0	,

LOQ = Limit of Quantitation. ND = Not Detected. Results reported on dry-weight basis.

Terpene Profile

Method: GC-FID · Headspace Sampling

Terpene	Result (%)	mg/g	Aroma Note
Myrcene	0.680	6.80	Earthy, mango
Limonene	0.460	4.60	Citrus, lemon
Caryophyllene	0.300	3.00	Spicy, peppery
Humulene	0.220	2.20	Hoppy, woody
Camphene	0.130	1.30	Damp woodland
Total Terpenes	1.79	17.90	Earthy, mango

Safety & Contaminant Screen

All categories tested per Cal. BCC Title 16 §5719

Pesticides & Plant Growth Regulators

66-analyte panel · LC-MS/MS & GC-MS/MS

PASS

Heavy Metals

Pb, Cd, As, Hg · ICP-MS

PASS

Microbial Impurities

Salmonella, STEC, Aspergillus spp. · qPCR

PASS

Mycotoxins

Aflatoxins B1, B2, G1, G2 · Ochratoxin A · LC-MS/MS

PASS

Residual Solvents

Cat I & II solvents · GC-MS Headspace

PASS

Foreign Matter

Visual + microscopic inspection

PASS

Filth & Foreign Materials

Insect frag., mammalian excreta, hair, mould

PASS

Water Activity & Moisture

aw meter · oven loss-on-drying

PASS

Quality Metrics

Stability & storage indicators

Moisture Content

9.5%

Water Activity (aw)

0.54

Density (g/mL)

0.40

Phenotype Consistency & Stabilization Line

RKS internal selection criteria · not tested by lab

Phenotype Consistency

The values reported on this Certificate reflect the top-performing phenotype(RW-Pheno #1) selected from a 13-plant cohort of the RKS Red Widow Feminized line. Across three production cycles, individual plants from this seed line have fallen within a 10–15% variation envelope on total cannabinoids and total terpene yield when grown under comparable indoor conditions.

Cohort variation (n=13)Reference pheno = THC 25.0%

22.3% (low)27.3% (high)

Note: phenotype expression is subject to environmental conditions (light, nutrients, VPD, training). These figures are not a guarantee of grow outcome.

Stabilization Status

FEMINIZED PHOTOPERIOD

This line has been stabilized through successive inbred generations with phenotype-driven selection. Trait-locking criteria for the RW-Pheno #1 selection:

Balanced terpene expression (no single >50% of profile)
Predictable hybrid effect profile
Cohort variation ≤ 12% on total cannabinoids
Trichome density ≥ 28%
Resistance to common mildew strains
Flower window 8–10 weeks

Expected Grow Outcomes

RKS internal cultivation reference · standard indoor SOP

⏱

Flower Time

8 – 10

weeks

🏠

Yield (Indoor)

500

g / m²

☀

Yield (Outdoor)

600

g / plant

↕

Plant Height

160

cm indoor

⚒

Difficulty

Easy

beginner+

Reference outcomes assume standard cultivation conditions: 600–900 PPFD, VPD 1.0–1.4 kPa, mineral-fed coco substrate, 18/6 veg · 12/12 flower. Outcomes will vary with cultivator skill, environment, and phenotype selection.

♛ RKS Genetics Authority Statement

The genetic line described in this reference benchmark was bred, selected, and stabilized by the Royal King Seeds Breeding Team. The cannabinoid and terpene ranges shown are aggregated from breeder documentation and publicly-reported lab data for this strain family, not from an independent ISO 17025 certificate of analysis. The data is published as a reference benchmark for the RKS Red Widowstabilized line, a documented standard against which every seed in this lot has been bred to perform.

ROYAL KING GENETICS

Breeding Team Documentation

Reference Benchmark

Not an independent ISO 17025 COA

Reference Method Notes

Standard cannabis-analysis methods used in published third-party lab data

HPLC-DAD

Cannabinoid potency by reverse-phase HPLC with diode array detection. SOP-CAN-101 Rev 4.

GC-FID Headspace

Terpene quantification · 38-analyte target list. SOP-TER-204 Rev 2.

LC-MS/MS & GC-MS/MS

Pesticide and mycotoxin screening with QuEChERS prep. SOP-PES-310 Rev 5.

ICP-MS

Heavy metals (Pb, Cd, As, Hg) digested via microwave HNO₃. SOP-MET-115 Rev 3.

qPCR Microbiology

PathoSEEK® assay for Salmonella, STEC, and Aspergillus. SOP-MIC-401 Rev 2.

GC-MS Headspace (Solvents)

Cat I & II residual solvents per USP <467>. SOP-RSV-220 Rev 3.

Laboratory Authorization

Document validity: 12 months from date of issue

Royal King Genetics Lab Team

Quality Analysis Team

Royal King Genetics QA

Quality Assurance Lead

Disclaimer of variation · Results published in this Certificate represent a selected phenotype of the RKS Red Widow Feminized stabilized line under controlled indoor conditions. Individual seedlings may produce plants that vary by approximately 10–15% on total cannabinoids and terpene output, and may express alternate phenotypes within the line. Final cannabinoid and terpene expression is subject to cultivator inputs, lighting, substrate, nutrient regime, environment, and training technique. This document is intended as a reference benchmark of the genetic potential of the RKS Red Widowline, not as a guarantee of any individual plant’s outcome.

Note for seed buyers · This Certificate analyzes mature, cured flower from a selected reference plant cultivated by the RKS breeding team under controlled conditions. Seed-grown plants may vary slightly from the tested phenotype depending on environment, nutrient regime, and grower input. For best expression of the RW-Pheno #1profile, follow the cultivation reference outlined in the “Expected Grow Outcomes” section above.

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